Primer Details for TRINITY_DN12888_c6_g1_i1 | |||
|---|---|---|---|
| (TTA)7: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCTCTCTGTGAGTGGAAA | TCTAACTCACAACCGATAGCAA | Start: 22 |
| Tm | 58.576 | 57.206 | End: 168 |
| Size | 20 | 22 | 147 |
| Primer 2 details | |||
| Sequence | TTGGCTCTCTGTGAGTGGAA | TCTAACTCACAACCGATAGCAA | Start: 21 |
| Tm | 58.576 | 57.206 | End: 168 |
| Size | 20 | 22 | 148 |
| Primer 3 details | |||
| Sequence | TGGCTCTCTGTGAGTGGAAA | CACAACCGATAGCAATTTAGAGACC | Start: 22 |
| Tm | 58.576 | 59.993 | End: 161 |
| Size | 20 | 25 | 140 |
| Primer 4 details | |||
| Sequence | TTGGCTCTCTGTGAGTGGAA | CACAACCGATAGCAATTTAGAGACC | Start: 21 |
| Tm | 58.576 | 59.993 | End: 161 |
| Size | 20 | 25 | 141 |
| Primer 5 details | |||
| Sequence | ATTGGCTCTCTGTGAGTGGA | TCTAACTCACAACCGATAGCAA | Start: 20 |
| Tm | 58.351 | 57.206 | End: 168 |
| Size | 20 | 22 | 149 |
