ssr_primer
Primer Details for TRINITY_DN12888_c4_g1_i1 |
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| (TTA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTTGGCTTGCTAGGTCC | CGTTCATGGGCTCAGTGACT | Start: 333 |
| Tm | 59.963 | 60.037 | End: 592 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
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| Sequence | ACACTTGGCTTGCTAGGTCC | AAAGATGCATGTGCGGTTGG | Start: 333 |
| Tm | 59.963 | 59.756 | End: 446 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
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| Sequence | CCAACCGCACATGCATCTTT | CGTTCATGGGCTCAGTGACT | Start: 427 |
| Tm | 59.756 | 60.037 | End: 592 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
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| Sequence | ACACTTGGCTTGCTAGGTCC | ACGTTCATGGGCTCAGTGAC | Start: 333 |
| Tm | 59.963 | 60.321 | End: 593 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
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| Sequence | CACTTGGCTTGCTAGGTCCT | CGTTCATGGGCTCAGTGACT | Start: 334 |
| Tm | 59.674 | 60.037 | End: 592 |
| Size | 20 | 20 | 259 |
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