ssr_primer
Primer Details for TRINITY_DN12883_c1_g3_i5 |
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| (GAA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGCCATTTACGGACAGAG | GCTGCTCGAACCTTCATCCT | Start: 1467 |
| Tm | 60.108 | 60.108 | End: 1668 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
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| Sequence | ACGGCCATTTACGGACAGAG | GGATTGGGAGATTCGGGTCC | Start: 1467 |
| Tm | 60.108 | 59.891 | End: 1638 |
| Size | 20 | 20 | 172 |
| Primer 3 details |
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| Sequence | ACGAAGGCATTACAGCACCA | ACTGCCACTTGTCTTCAGCA | Start: 773 |
| Tm | 59.964 | 59.819 | End: 880 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
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| Sequence | ACCACGAAGGCATTACAGCA | ACTGCCACTTGTCTTCAGCA | Start: 770 |
| Tm | 59.964 | 59.819 | End: 880 |
| Size | 20 | 20 | 111 |
| Primer 5 details |
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| Sequence | ACGGCCATTTACGGACAGAG | AGAGCCTCTTCACTTCCCCT | Start: 1467 |
| Tm | 60.108 | 59.882 | End: 1601 |
| Size | 20 | 20 | 135 |
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