ssr_primer
Primer Details for TRINITY_DN12870_c5_g1_i5 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCTAGCATGGATGGCCTC | CATCAGGTGCTGGAGCTTCA | Start: 1336 |
| Tm | 59.964 | 60.036 | End: 1466 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | TGGATCATCACACCACACGG | AATCAGGACTGTGTCAGCGG | Start: 3640 |
| Tm | 60.036 | 60.037 | End: 3842 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
|---|
| Sequence | AAGGCGTTTACGGTTACGGT | ACCGGTCTCCCCTGTCATTA | Start: 2421 |
| Tm | 59.967 | 59.958 | End: 2674 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | AAGGCGTTTACGGTTACGGT | TACCGGTCTCCCCTGTCATT | Start: 2421 |
| Tm | 59.967 | 59.958 | End: 2675 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | GGCCGAGATCGTTTTGTTCG | AATCAGGACTGTGTCAGCGG | Start: 3709 |
| Tm | 59.905 | 60.037 | End: 3842 |
| Size | 20 | 20 | 134 |
(TC)7gtcaaagaaaaaacagcacgaaatgt(AG)7attataggaagcagaagaaatatagagagaaaaaaggaaagg(GAA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCTAGCATGGATGGCCTC | CATCAGGTGCTGGAGCTTCA | Start: 1336 |
| Tm | 59.964 | 60.036 | End: 1466 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | TGGATCATCACACCACACGG | AATCAGGACTGTGTCAGCGG | Start: 3640 |
| Tm | 60.036 | 60.037 | End: 3842 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
|---|
| Sequence | AAGGCGTTTACGGTTACGGT | ACCGGTCTCCCCTGTCATTA | Start: 2421 |
| Tm | 59.967 | 59.958 | End: 2674 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | AAGGCGTTTACGGTTACGGT | TACCGGTCTCCCCTGTCATT | Start: 2421 |
| Tm | 59.967 | 59.958 | End: 2675 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | GGCCGAGATCGTTTTGTTCG | AATCAGGACTGTGTCAGCGG | Start: 3709 |
| Tm | 59.905 | 60.037 | End: 3842 |
| Size | 20 | 20 | 134 |
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