ssr_primer
Primer Details for TRINITY_DN12866_c2_g1_i5 |
|---|
| (AAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAGCGAAAACTGTTGCTC | TTCTTCCTGGTACGCACGAC | Start: 5886 |
| Tm | 59.971 | 60.039 | End: 6030 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
|---|
| Sequence | CTGCAGCGAAAACTGTTGCT | TTCTTCCTGGTACGCACGAC | Start: 5885 |
| Tm | 59.97 | 60.039 | End: 6030 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | ATGTCAACCATGCCCACCAT | ATTCCTCTCGGGGGATCCAA | Start: 1359 |
| Tm | 59.958 | 60.03 | End: 1470 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | TTTCCGGGACATCAATGCCA | AATGGCCTGCAGTTACCCTC | Start: 1566 |
| Tm | 59.961 | 60.034 | End: 1751 |
| Size | 20 | 20 | 186 |
| Primer 5 details |
|---|
| Sequence | TTTCCGGGACATCAATGCCA | GAATGGCCTGCAGTTACCCT | Start: 1566 |
| Tm | 59.961 | 60.034 | End: 1752 |
| Size | 20 | 20 | 187 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAGCGAAAACTGTTGCTC | TTCTTCCTGGTACGCACGAC | Start: 5886 |
| Tm | 59.971 | 60.039 | End: 6030 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
|---|
| Sequence | CTGCAGCGAAAACTGTTGCT | TTCTTCCTGGTACGCACGAC | Start: 5885 |
| Tm | 59.97 | 60.039 | End: 6030 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | ATGTCAACCATGCCCACCAT | ATTCCTCTCGGGGGATCCAA | Start: 1359 |
| Tm | 59.958 | 60.03 | End: 1470 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | TTTCCGGGACATCAATGCCA | AATGGCCTGCAGTTACCCTC | Start: 1566 |
| Tm | 59.961 | 60.034 | End: 1751 |
| Size | 20 | 20 | 186 |
| Primer 5 details |
|---|
| Sequence | TTTCCGGGACATCAATGCCA | GAATGGCCTGCAGTTACCCT | Start: 1566 |
| Tm | 59.961 | 60.034 | End: 1752 |
| Size | 20 | 20 | 187 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAGCGAAAACTGTTGCTC | TTCTTCCTGGTACGCACGAC | Start: 5886 |
| Tm | 59.971 | 60.039 | End: 6030 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
|---|
| Sequence | CTGCAGCGAAAACTGTTGCT | TTCTTCCTGGTACGCACGAC | Start: 5885 |
| Tm | 59.97 | 60.039 | End: 6030 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | ATGTCAACCATGCCCACCAT | ATTCCTCTCGGGGGATCCAA | Start: 1359 |
| Tm | 59.958 | 60.03 | End: 1470 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | TTTCCGGGACATCAATGCCA | AATGGCCTGCAGTTACCCTC | Start: 1566 |
| Tm | 59.961 | 60.034 | End: 1751 |
| Size | 20 | 20 | 186 |
| Primer 5 details |
|---|
| Sequence | TTTCCGGGACATCAATGCCA | GAATGGCCTGCAGTTACCCT | Start: 1566 |
| Tm | 59.961 | 60.034 | End: 1752 |
| Size | 20 | 20 | 187 |
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