ssr_primer
Primer Details for TRINITY_DN12866_c1_g1_i3 |
|---|
| (TCT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCTCCGCCACTATTACCG | TGGGTCGACAAAGACGAAGG | Start: 285 |
| Tm | 59.968 | 59.967 | End: 564 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | TGCCACTAACAACTGCGCTA | CGGTAATAGTGGCGGAGGAC | Start: 139 |
| Tm | 59.966 | 59.968 | End: 304 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | CACGCAGAAGAGGAAGGGTT | GTTGGATGGGGCTTGCTACT | Start: 2301 |
| Tm | 59.965 | 60.034 | End: 2486 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AGTAGCAAGCCCCATCCAAC | GCCTTCATGGGGTCCTTTGA | Start: 2467 |
| Tm | 60.034 | 59.96 | End: 2717 |
| Size | 20 | 20 | 251 |
| Primer 5 details |
|---|
| Sequence | AGTAGCAAGCCCCATCCAAC | CTGCCTTCATGGGGTCCTTT | Start: 2467 |
| Tm | 60.034 | 59.96 | End: 2719 |
| Size | 20 | 20 | 253 |
(CT)9(AT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCTCCGCCACTATTACCG | TGGGTCGACAAAGACGAAGG | Start: 285 |
| Tm | 59.968 | 59.967 | End: 564 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | TGCCACTAACAACTGCGCTA | CGGTAATAGTGGCGGAGGAC | Start: 139 |
| Tm | 59.966 | 59.968 | End: 304 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | CACGCAGAAGAGGAAGGGTT | GTTGGATGGGGCTTGCTACT | Start: 2301 |
| Tm | 59.965 | 60.034 | End: 2486 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AGTAGCAAGCCCCATCCAAC | GCCTTCATGGGGTCCTTTGA | Start: 2467 |
| Tm | 60.034 | 59.96 | End: 2717 |
| Size | 20 | 20 | 251 |
| Primer 5 details |
|---|
| Sequence | AGTAGCAAGCCCCATCCAAC | CTGCCTTCATGGGGTCCTTT | Start: 2467 |
| Tm | 60.034 | 59.96 | End: 2719 |
| Size | 20 | 20 | 253 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCTCCGCCACTATTACCG | TGGGTCGACAAAGACGAAGG | Start: 285 |
| Tm | 59.968 | 59.967 | End: 564 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | TGCCACTAACAACTGCGCTA | CGGTAATAGTGGCGGAGGAC | Start: 139 |
| Tm | 59.966 | 59.968 | End: 304 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | CACGCAGAAGAGGAAGGGTT | GTTGGATGGGGCTTGCTACT | Start: 2301 |
| Tm | 59.965 | 60.034 | End: 2486 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AGTAGCAAGCCCCATCCAAC | GCCTTCATGGGGTCCTTTGA | Start: 2467 |
| Tm | 60.034 | 59.96 | End: 2717 |
| Size | 20 | 20 | 251 |
| Primer 5 details |
|---|
| Sequence | AGTAGCAAGCCCCATCCAAC | CTGCCTTCATGGGGTCCTTT | Start: 2467 |
| Tm | 60.034 | 59.96 | End: 2719 |
| Size | 20 | 20 | 253 |
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