ssr_primer
Primer Details for TRINITY_DN12862_c2_g1_i8 |
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| (TA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAACGCATTCCTTGTCGGA | CTCTCCCCTCCCTAACCACA | Start: 133 |
| Tm | 59.966 | 59.957 | End: 328 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
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| Sequence | TGTGGTTAGGGAGGGGAGAG | ATTTGAGCGGTGACATGGGT | Start: 309 |
| Tm | 59.957 | 59.962 | End: 513 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
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| Sequence | TGTGGTTAGGGAGGGGAGAG | GCGCTTGTTGAGTGTGAAGG | Start: 309 |
| Tm | 59.957 | 60.041 | End: 544 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
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| Sequence | TGTGGTTAGGGAGGGGAGAG | GGCGCTTGTTGAGTGTGAAG | Start: 309 |
| Tm | 59.957 | 60.041 | End: 545 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
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| Sequence | TCAGGCGTGAAGTCAACGAA | CACACTGGGTCTTCTTCGCT | Start: 2448 |
| Tm | 59.897 | 59.966 | End: 2589 |
| Size | 20 | 20 | 142 |
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