ssr_primer
Primer Details for TRINITY_DN12859_c5_g1_i22 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCACTCACTGGAGCTCAA | AAGATCCCGACCAAACCGAC | Start: 504 |
| Tm | 59.966 | 60.037 | End: 604 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ATCACTGCACTCACTGGAGC | AAGATCCCGACCAAACCGAC | Start: 500 |
| Tm | 60.037 | 60.037 | End: 604 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | GTCGGTTTGGTCGGGATCTT | ATACGTTTTCAGCGCTCCCA | Start: 585 |
| Tm | 60.037 | 60.037 | End: 822 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | GCAGCGTTTGTCACTCCAAG | AGGGTGTTTAGGGCTCGTTG | Start: 1219 |
| Tm | 60.041 | 59.964 | End: 1327 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
|---|
| Sequence | ACAGTTTTGTTTGCCCTCGC | TTGAGCTCCAGTGAGTGCAG | Start: 369 |
| Tm | 59.898 | 59.966 | End: 523 |
| Size | 20 | 20 | 155 |
(TG)9tatgtatacatgtgtattctgt(TG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCACTCACTGGAGCTCAA | AAGATCCCGACCAAACCGAC | Start: 504 |
| Tm | 59.966 | 60.037 | End: 604 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ATCACTGCACTCACTGGAGC | AAGATCCCGACCAAACCGAC | Start: 500 |
| Tm | 60.037 | 60.037 | End: 604 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | GTCGGTTTGGTCGGGATCTT | ATACGTTTTCAGCGCTCCCA | Start: 585 |
| Tm | 60.037 | 60.037 | End: 822 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | GCAGCGTTTGTCACTCCAAG | AGGGTGTTTAGGGCTCGTTG | Start: 1219 |
| Tm | 60.041 | 59.964 | End: 1327 |
| Size | 20 | 20 | 109 |
| Primer 5 details |
|---|
| Sequence | ACAGTTTTGTTTGCCCTCGC | TTGAGCTCCAGTGAGTGCAG | Start: 369 |
| Tm | 59.898 | 59.966 | End: 523 |
| Size | 20 | 20 | 155 |
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