ssr_primer
Primer Details for TRINITY_DN12853_c0_g1_i5 |
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| (TTC)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCACCATCTTTCAACCCA | TCTAGTTTTGCTCCGGCCAG | Start: 2817 |
| Tm | 59.886 | 60.036 | End: 2960 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
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| Sequence | CCCCACCATCTTTCAACCCA | ATGTGCAGAGTGGGACCTTG | Start: 2817 |
| Tm | 59.886 | 59.963 | End: 2930 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
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| Sequence | ACATTCTTCCCGAGCCGAAG | CTCCAGGTAACATCTCGGGC | Start: 486 |
| Tm | 60.108 | 59.895 | End: 699 |
| Size | 20 | 20 | 214 |
| Primer 4 details |
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| Sequence | GATGGCGGAAGAGAGGATGG | TCATCCATGGCCAAGAGACG | Start: 3250 |
| Tm | 59.965 | 59.821 | End: 3391 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
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| Sequence | CCAGCCCGTGTAGAACTCTG | GTGGCAATGCTGTGTTCTCG | Start: 1715 |
| Tm | 60.109 | 60.11 | End: 1845 |
| Size | 20 | 20 | 131 |
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