ssr_primer
Primer Details for TRINITY_DN12850_c0_g2_i5 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGGGAGACATATGGAACG | GGCATTATGGCACTCGCAAG | Start: 1342 |
| Tm | 59.966 | 59.97 | End: 1584 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | CGGGGGAGACATATGGAACG | AGTGGTTGCTTGGACTAGGC | Start: 1342 |
| Tm | 59.966 | 59.963 | End: 1527 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
|---|
| Sequence | CTTCGCCATTGTCATCTGCG | CCAACCCTTCTCAGCTCTCG | Start: 5901 |
| Tm | 59.973 | 60.108 | End: 6155 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | GTCACAGCAACGGCAACTTT | AAGACAACGTAAGGGCAGGG | Start: 5233 |
| Tm | 59.899 | 59.964 | End: 5351 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | TACTGCCATCCTCCCATCGA | CGTTCCATATGTCTCCCCCG | Start: 1133 |
| Tm | 60.106 | 59.966 | End: 1361 |
| Size | 20 | 20 | 229 |
(AAT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGGGAGACATATGGAACG | GGCATTATGGCACTCGCAAG | Start: 1342 |
| Tm | 59.966 | 59.97 | End: 1584 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | CGGGGGAGACATATGGAACG | AGTGGTTGCTTGGACTAGGC | Start: 1342 |
| Tm | 59.966 | 59.963 | End: 1527 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
|---|
| Sequence | CTTCGCCATTGTCATCTGCG | CCAACCCTTCTCAGCTCTCG | Start: 5901 |
| Tm | 59.973 | 60.108 | End: 6155 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | GTCACAGCAACGGCAACTTT | AAGACAACGTAAGGGCAGGG | Start: 5233 |
| Tm | 59.899 | 59.964 | End: 5351 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | TACTGCCATCCTCCCATCGA | CGTTCCATATGTCTCCCCCG | Start: 1133 |
| Tm | 60.106 | 59.966 | End: 1361 |
| Size | 20 | 20 | 229 |
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