ssr_primer
Primer Details for TRINITY_DN12843_c1_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGATTCCGGCGAGTCAAA | TACTGCTGTTTCCCACCGAC | Start: 1124 |
| Tm | 60.038 | 59.966 | End: 1239 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TACTGCTGTTTCCCACCGAC | Start: 1069 |
| Tm | 59.895 | 59.966 | End: 1239 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TTCGATTCCGGCGAGTCAAA | TTTCCCACCGACGGAAATGT | Start: 1124 |
| Tm | 60.038 | 59.891 | End: 1231 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TTTCCCACCGACGGAAATGT | Start: 1069 |
| Tm | 59.895 | 59.891 | End: 1231 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | GTCAATTGCCGGCGACATTT | ACTTAGAGCCGCCAACATCC | Start: 1147 |
| Tm | 60.109 | 60.108 | End: 1415 |
| Size | 20 | 20 | 269 |
(TG)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGATTCCGGCGAGTCAAA | TACTGCTGTTTCCCACCGAC | Start: 1124 |
| Tm | 60.038 | 59.966 | End: 1239 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TACTGCTGTTTCCCACCGAC | Start: 1069 |
| Tm | 59.895 | 59.966 | End: 1239 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TTCGATTCCGGCGAGTCAAA | TTTCCCACCGACGGAAATGT | Start: 1124 |
| Tm | 60.038 | 59.891 | End: 1231 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TTTCCCACCGACGGAAATGT | Start: 1069 |
| Tm | 59.895 | 59.891 | End: 1231 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | GTCAATTGCCGGCGACATTT | ACTTAGAGCCGCCAACATCC | Start: 1147 |
| Tm | 60.109 | 60.108 | End: 1415 |
| Size | 20 | 20 | 269 |
(A)10ggggg(A)11gacaaaagattgtgtctacacatatgatcatggatggtgaaaatgggatccgaagacctaattttccattacaattgttggagaagaaaaag(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGATTCCGGCGAGTCAAA | TACTGCTGTTTCCCACCGAC | Start: 1124 |
| Tm | 60.038 | 59.966 | End: 1239 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TACTGCTGTTTCCCACCGAC | Start: 1069 |
| Tm | 59.895 | 59.966 | End: 1239 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TTCGATTCCGGCGAGTCAAA | TTTCCCACCGACGGAAATGT | Start: 1124 |
| Tm | 60.038 | 59.891 | End: 1231 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TTTCCCACCGACGGAAATGT | Start: 1069 |
| Tm | 59.895 | 59.891 | End: 1231 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | GTCAATTGCCGGCGACATTT | ACTTAGAGCCGCCAACATCC | Start: 1147 |
| Tm | 60.109 | 60.108 | End: 1415 |
| Size | 20 | 20 | 269 |
(GGT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGATTCCGGCGAGTCAAA | TACTGCTGTTTCCCACCGAC | Start: 1124 |
| Tm | 60.038 | 59.966 | End: 1239 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TACTGCTGTTTCCCACCGAC | Start: 1069 |
| Tm | 59.895 | 59.966 | End: 1239 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TTCGATTCCGGCGAGTCAAA | TTTCCCACCGACGGAAATGT | Start: 1124 |
| Tm | 60.038 | 59.891 | End: 1231 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TTTCCCACCGACGGAAATGT | Start: 1069 |
| Tm | 59.895 | 59.891 | End: 1231 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | GTCAATTGCCGGCGACATTT | ACTTAGAGCCGCCAACATCC | Start: 1147 |
| Tm | 60.109 | 60.108 | End: 1415 |
| Size | 20 | 20 | 269 |
(T)10ctactcattcattttctcactaaatatacatatcatattaaatatgagttatgcaattttaccacaataggatttagaggtatgtattgagaag(AGA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGATTCCGGCGAGTCAAA | TACTGCTGTTTCCCACCGAC | Start: 1124 |
| Tm | 60.038 | 59.966 | End: 1239 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TACTGCTGTTTCCCACCGAC | Start: 1069 |
| Tm | 59.895 | 59.966 | End: 1239 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TTCGATTCCGGCGAGTCAAA | TTTCCCACCGACGGAAATGT | Start: 1124 |
| Tm | 60.038 | 59.891 | End: 1231 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TTTCCCACCGACGGAAATGT | Start: 1069 |
| Tm | 59.895 | 59.891 | End: 1231 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | GTCAATTGCCGGCGACATTT | ACTTAGAGCCGCCAACATCC | Start: 1147 |
| Tm | 60.109 | 60.108 | End: 1415 |
| Size | 20 | 20 | 269 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGATTCCGGCGAGTCAAA | TACTGCTGTTTCCCACCGAC | Start: 1124 |
| Tm | 60.038 | 59.966 | End: 1239 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TACTGCTGTTTCCCACCGAC | Start: 1069 |
| Tm | 59.895 | 59.966 | End: 1239 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TTCGATTCCGGCGAGTCAAA | TTTCCCACCGACGGAAATGT | Start: 1124 |
| Tm | 60.038 | 59.891 | End: 1231 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GGTCGGAGCAATCGGAATCT | TTTCCCACCGACGGAAATGT | Start: 1069 |
| Tm | 59.895 | 59.891 | End: 1231 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | GTCAATTGCCGGCGACATTT | ACTTAGAGCCGCCAACATCC | Start: 1147 |
| Tm | 60.109 | 60.108 | End: 1415 |
| Size | 20 | 20 | 269 |
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