ssr_primer
Primer Details for TRINITY_DN12837_c2_g4_i3 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCGAGAGATCGAGGCAAA | GCCACTTCGTTGCACCAATT | Start: 64 |
| Tm | 59.899 | 59.968 | End: 195 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | CTAGATCGCATGCCAAGGGT | AAACAGAAAGCAGCAGCAGC | Start: 916 |
| Tm | 59.893 | 59.968 | End: 1173 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | ACTAGATCGCATGCCAAGGG | AAACAGAAAGCAGCAGCAGC | Start: 915 |
| Tm | 59.893 | 59.968 | End: 1173 |
| Size | 20 | 20 | 259 |
| Primer 4 details |
|---|
| Sequence | GAGCGAGAGATCGAGGCAAA | AAACTGCCACTTCGTTGCAC | Start: 64 |
| Tm | 59.899 | 59.899 | End: 200 |
| Size | 20 | 20 | 137 |
| Primer 5 details |
|---|
| Sequence | GAGCGAGAGATCGAGGCAAA | CTGGGGAGATTGCAGAGGTG | Start: 64 |
| Tm | 59.899 | 60.107 | End: 231 |
| Size | 20 | 20 | 168 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCGAGAGATCGAGGCAAA | GCCACTTCGTTGCACCAATT | Start: 64 |
| Tm | 59.899 | 59.968 | End: 195 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | CTAGATCGCATGCCAAGGGT | AAACAGAAAGCAGCAGCAGC | Start: 916 |
| Tm | 59.893 | 59.968 | End: 1173 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | ACTAGATCGCATGCCAAGGG | AAACAGAAAGCAGCAGCAGC | Start: 915 |
| Tm | 59.893 | 59.968 | End: 1173 |
| Size | 20 | 20 | 259 |
| Primer 4 details |
|---|
| Sequence | GAGCGAGAGATCGAGGCAAA | AAACTGCCACTTCGTTGCAC | Start: 64 |
| Tm | 59.899 | 59.899 | End: 200 |
| Size | 20 | 20 | 137 |
| Primer 5 details |
|---|
| Sequence | GAGCGAGAGATCGAGGCAAA | CTGGGGAGATTGCAGAGGTG | Start: 64 |
| Tm | 59.899 | 60.107 | End: 231 |
| Size | 20 | 20 | 168 |
(TGC)6tttctgtttgtttgtttgtttaaagcttggtgtttttagcataaaggaaacgtgg(GTT)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCGAGAGATCGAGGCAAA | GCCACTTCGTTGCACCAATT | Start: 64 |
| Tm | 59.899 | 59.968 | End: 195 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | CTAGATCGCATGCCAAGGGT | AAACAGAAAGCAGCAGCAGC | Start: 916 |
| Tm | 59.893 | 59.968 | End: 1173 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | ACTAGATCGCATGCCAAGGG | AAACAGAAAGCAGCAGCAGC | Start: 915 |
| Tm | 59.893 | 59.968 | End: 1173 |
| Size | 20 | 20 | 259 |
| Primer 4 details |
|---|
| Sequence | GAGCGAGAGATCGAGGCAAA | AAACTGCCACTTCGTTGCAC | Start: 64 |
| Tm | 59.899 | 59.899 | End: 200 |
| Size | 20 | 20 | 137 |
| Primer 5 details |
|---|
| Sequence | GAGCGAGAGATCGAGGCAAA | CTGGGGAGATTGCAGAGGTG | Start: 64 |
| Tm | 59.899 | 60.107 | End: 231 |
| Size | 20 | 20 | 168 |
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