ssr_primer
Primer Details for TRINITY_DN12833_c1_g1_i1 |
|---|
| (CT)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCCATCCCACCACATCCC | GGCAGTGAGAAGCAGAGTGT | Start: 140 |
| Tm | 60.107 | 59.965 | End: 291 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | ACACTCTGCTTCTCACTGCC | GTGGTAGTGGCAGTGTTGGA | Start: 272 |
| Tm | 59.965 | 59.892 | End: 522 |
| Size | 20 | 20 | 251 |
| Primer 3 details |
|---|
| Sequence | ACACTCTGCTTCTCACTGCC | AGTGGTAGTGGCAGTGTTGG | Start: 272 |
| Tm | 59.965 | 59.891 | End: 523 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | ACACTCTGCTTCTCACTGCC | ACTCAAGCTTGGTGGACAGG | Start: 272 |
| Tm | 59.965 | 59.891 | End: 547 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
|---|
| Sequence | TGATGGCGAGATCAATGGGG | GTGTAGAGCACCGTGAGCTT | Start: 628 |
| Tm | 59.891 | 60.038 | End: 895 |
| Size | 20 | 20 | 268 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCCATCCCACCACATCCC | GGCAGTGAGAAGCAGAGTGT | Start: 140 |
| Tm | 60.107 | 59.965 | End: 291 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | ACACTCTGCTTCTCACTGCC | GTGGTAGTGGCAGTGTTGGA | Start: 272 |
| Tm | 59.965 | 59.892 | End: 522 |
| Size | 20 | 20 | 251 |
| Primer 3 details |
|---|
| Sequence | ACACTCTGCTTCTCACTGCC | AGTGGTAGTGGCAGTGTTGG | Start: 272 |
| Tm | 59.965 | 59.891 | End: 523 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | ACACTCTGCTTCTCACTGCC | ACTCAAGCTTGGTGGACAGG | Start: 272 |
| Tm | 59.965 | 59.891 | End: 547 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
|---|
| Sequence | TGATGGCGAGATCAATGGGG | GTGTAGAGCACCGTGAGCTT | Start: 628 |
| Tm | 59.891 | 60.038 | End: 895 |
| Size | 20 | 20 | 268 |
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