ssr_primer
Primer Details for TRINITY_DN12830_c0_g7_i3 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGGCTCCCCTTGGATTA | TGCAGCTCAAGCCTGAAGAA | Start: 1532 |
| Tm | 59.954 | 59.891 | End: 1653 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
|---|
| Sequence | TCCAGGCTCCCCTTGGATTA | AAGTTTCTGGCATCGTGGGT | Start: 1532 |
| Tm | 59.954 | 59.89 | End: 1635 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | GCATTCACGTGCGTTCTCAG | TTGATTTTTGCAGCGGGCTC | Start: 311 |
| Tm | 60.179 | 60.038 | End: 551 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | GCACGATGCTGCAAACTTGA | CTGAGAACGCACGTGAATGC | Start: 197 |
| Tm | 60.04 | 60.179 | End: 330 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | GCGGTAGGCGGGTATGTTAA | CTGAGAACGCACGTGAATGC | Start: 176 |
| Tm | 59.895 | 60.179 | End: 330 |
| Size | 20 | 20 | 155 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGGCTCCCCTTGGATTA | TGCAGCTCAAGCCTGAAGAA | Start: 1532 |
| Tm | 59.954 | 59.891 | End: 1653 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
|---|
| Sequence | TCCAGGCTCCCCTTGGATTA | AAGTTTCTGGCATCGTGGGT | Start: 1532 |
| Tm | 59.954 | 59.89 | End: 1635 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | GCATTCACGTGCGTTCTCAG | TTGATTTTTGCAGCGGGCTC | Start: 311 |
| Tm | 60.179 | 60.038 | End: 551 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | GCACGATGCTGCAAACTTGA | CTGAGAACGCACGTGAATGC | Start: 197 |
| Tm | 60.04 | 60.179 | End: 330 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | GCGGTAGGCGGGTATGTTAA | CTGAGAACGCACGTGAATGC | Start: 176 |
| Tm | 59.895 | 60.179 | End: 330 |
| Size | 20 | 20 | 155 |
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