Primer Details for TRINITY_DN12829_c1_g1_i1 |
|---|
| (AAG)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCACTAGGTTTTAGAAGGAACTT | TGACCAACCCACCAACATGG | Start: 6 |
| Tm | 57.241 | 60.469 | End: 155 |
| Size | 24 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | ATCCACTAGGTTTTAGAAGGAACT | TGACCAACCCACCAACATGG | Start: 5 |
| Tm | 57.046 | 60.469 | End: 155 |
| Size | 24 | 20 | 151 |
| Primer 3 details |
|---|
| Sequence | TCCACTAGGTTTTAGAAGGAACTTT | TGACCAACCCACCAACATGG | Start: 6 |
| Tm | 57.855 | 60.469 | End: 155 |
| Size | 25 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AGAATCCACTAGGTTTTAGAAGGAACT | TGACCAACCCACCAACATGG | Start: 2 |
| Tm | 59.762 | 60.469 | End: 155 |
| Size | 27 | 20 | 154 |
| Primer 5 details |
|---|
| Sequence | ATCCACTAGGTTTTAGAAGGAACTT | TGACCAACCCACCAACATGG | Start: 5 |
| Tm | 57.669 | 60.469 | End: 155 |
| Size | 25 | 20 | 151 |
