ssr_primer
Primer Details for TRINITY_DN12829_c0_g6_i1 |
|---|
| (TTC)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAATGGGGATTGGTTCCGGT | CCTACATGCATGAAACGGCG | Start: 719 |
| Tm | 60.034 | 59.972 | End: 961 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | TCGTTCGAGTTGGGAATGGG | CCTACATGCATGAAACGGCG | Start: 706 |
| Tm | 60.037 | 59.972 | End: 961 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | CCTCGTCGGTGGATGATCAG | ACCGGAACCAATCCCCATTC | Start: 470 |
| Tm | 59.969 | 60.034 | End: 738 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
|---|
| Sequence | TTTCGGCCGGAGTCTTTACC | CCTACATGCATGAAACGGCG | Start: 820 |
| Tm | 60.037 | 59.972 | End: 961 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | CCTCGTCGGTGGATGATCAG | CCCATTCCCAACTCGAACGA | Start: 470 |
| Tm | 59.969 | 60.037 | End: 725 |
| Size | 20 | 20 | 256 |
(A)17gcacaaaaaaaccaactcccgatcagatcatgaagttggggtttggaaaaaataggatgagatttgaatggaatctctctagaagaagaaatgaac(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAATGGGGATTGGTTCCGGT | CCTACATGCATGAAACGGCG | Start: 719 |
| Tm | 60.034 | 59.972 | End: 961 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | TCGTTCGAGTTGGGAATGGG | CCTACATGCATGAAACGGCG | Start: 706 |
| Tm | 60.037 | 59.972 | End: 961 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | CCTCGTCGGTGGATGATCAG | ACCGGAACCAATCCCCATTC | Start: 470 |
| Tm | 59.969 | 60.034 | End: 738 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
|---|
| Sequence | TTTCGGCCGGAGTCTTTACC | CCTACATGCATGAAACGGCG | Start: 820 |
| Tm | 60.037 | 59.972 | End: 961 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | CCTCGTCGGTGGATGATCAG | CCCATTCCCAACTCGAACGA | Start: 470 |
| Tm | 59.969 | 60.037 | End: 725 |
| Size | 20 | 20 | 256 |
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