ssr_primer
Primer Details for TRINITY_DN12823_c4_g1_i29 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTATTGTACGCTGCCAGC | GATCCCTACACCTCCCCACT | Start: 5103 |
| Tm | 59.972 | 60.031 | End: 5252 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | AGTTCTTCGGTGGGCTCTTG | GCTGGCAGCGTACAATAAGC | Start: 4955 |
| Tm | 59.965 | 59.972 | End: 5122 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | CAAGTTCTTCGGTGGGCTCT | GCTGGCAGCGTACAATAAGC | Start: 4953 |
| Tm | 59.965 | 59.972 | End: 5122 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | GCTTATTGTACGCTGCCAGC | GATGAGACGAAGCACCACCA | Start: 5103 |
| Tm | 59.972 | 60.037 | End: 5307 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | GCTTATTGTACGCTGCCAGC | CACCTCCCCACTGCATTTCT | Start: 5103 |
| Tm | 59.972 | 59.961 | End: 5244 |
| Size | 20 | 20 | 142 |
(T)10attaattaattttgtgaattttctatttttctctt(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTATTGTACGCTGCCAGC | GATCCCTACACCTCCCCACT | Start: 5103 |
| Tm | 59.972 | 60.031 | End: 5252 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | AGTTCTTCGGTGGGCTCTTG | GCTGGCAGCGTACAATAAGC | Start: 4955 |
| Tm | 59.965 | 59.972 | End: 5122 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | CAAGTTCTTCGGTGGGCTCT | GCTGGCAGCGTACAATAAGC | Start: 4953 |
| Tm | 59.965 | 59.972 | End: 5122 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | GCTTATTGTACGCTGCCAGC | GATGAGACGAAGCACCACCA | Start: 5103 |
| Tm | 59.972 | 60.037 | End: 5307 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | GCTTATTGTACGCTGCCAGC | CACCTCCCCACTGCATTTCT | Start: 5103 |
| Tm | 59.972 | 59.961 | End: 5244 |
| Size | 20 | 20 | 142 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTATTGTACGCTGCCAGC | GATCCCTACACCTCCCCACT | Start: 5103 |
| Tm | 59.972 | 60.031 | End: 5252 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | AGTTCTTCGGTGGGCTCTTG | GCTGGCAGCGTACAATAAGC | Start: 4955 |
| Tm | 59.965 | 59.972 | End: 5122 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | CAAGTTCTTCGGTGGGCTCT | GCTGGCAGCGTACAATAAGC | Start: 4953 |
| Tm | 59.965 | 59.972 | End: 5122 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | GCTTATTGTACGCTGCCAGC | GATGAGACGAAGCACCACCA | Start: 5103 |
| Tm | 59.972 | 60.037 | End: 5307 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | GCTTATTGTACGCTGCCAGC | CACCTCCCCACTGCATTTCT | Start: 5103 |
| Tm | 59.972 | 59.961 | End: 5244 |
| Size | 20 | 20 | 142 |
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