ssr_primer
Primer Details for TRINITY_DN12796_c0_g1_i4 |
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| (AAAT)5ataaatatatatatacaaacacatgaatgaataatataataatg(ATA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCATCCATCAACACGGC | GTTGGGAGAACTCGAAGGCA | Start: 1562 |
| Tm | 59.97 | 59.965 | End: 1751 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
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| Sequence | CCAAACCGTGCAGAGACTCT | TGTTGACCCAGTTGCACACT | Start: 657 |
| Tm | 59.966 | 60.035 | End: 814 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
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| Sequence | GCATCATCCATCAACACGGC | GCTTTTGCCAGTCCTGAACG | Start: 1562 |
| Tm | 59.97 | 60.04 | End: 1723 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
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| Sequence | TTTCTCCCCTATCATCGCGC | GGGAGTTCAGGGTCTTTGGG | Start: 5292 |
| Tm | 59.966 | 59.962 | End: 5434 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
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| Sequence | GCACCAGCAAAAGAGGAAGC | GAGAACGCCCAGGCTAAAGT | Start: 4895 |
| Tm | 60.039 | 60.036 | End: 5151 |
| Size | 20 | 20 | 257 |
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