ssr_primer
Primer Details for TRINITY_DN12778_c1_g1_i2 |
|---|
| (TTC)5tttttctttcttcaacgtacactccatttcccctttctcttctcaaaccctaattctctctttaattaattcattaattcccctctcctt(TC)7acttcttccttcctcttccattaatggccgatt(CTC)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTCCGACGCTCTTTTCTT | GCGTCTGATAATCCAGCGGA | Start: 232 |
| Tm | 60.037 | 59.968 | End: 446 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | CCCTCCGACGCTCTTTTCTT | AGCGTCTGATAATCCAGCGG | Start: 232 |
| Tm | 60.037 | 59.967 | End: 447 |
| Size | 20 | 20 | 216 |
| Primer 3 details |
|---|
| Sequence | TCACTCTCACCGGATCCACT | GCGTCTGATAATCCAGCGGA | Start: 336 |
| Tm | 59.96 | 59.968 | End: 446 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | TCACTCTCACCGGATCCACT | AGCGTCTGATAATCCAGCGG | Start: 336 |
| Tm | 59.96 | 59.967 | End: 447 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | TTCCCTCACTCTCACCGGAT | GCGTCTGATAATCCAGCGGA | Start: 331 |
| Tm | 59.959 | 59.968 | End: 446 |
| Size | 20 | 20 | 116 |
(ACT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTCCGACGCTCTTTTCTT | GCGTCTGATAATCCAGCGGA | Start: 232 |
| Tm | 60.037 | 59.968 | End: 446 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | CCCTCCGACGCTCTTTTCTT | AGCGTCTGATAATCCAGCGG | Start: 232 |
| Tm | 60.037 | 59.967 | End: 447 |
| Size | 20 | 20 | 216 |
| Primer 3 details |
|---|
| Sequence | TCACTCTCACCGGATCCACT | GCGTCTGATAATCCAGCGGA | Start: 336 |
| Tm | 59.96 | 59.968 | End: 446 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | TCACTCTCACCGGATCCACT | AGCGTCTGATAATCCAGCGG | Start: 336 |
| Tm | 59.96 | 59.967 | End: 447 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | TTCCCTCACTCTCACCGGAT | GCGTCTGATAATCCAGCGGA | Start: 331 |
| Tm | 59.959 | 59.968 | End: 446 |
| Size | 20 | 20 | 116 |
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