ssr_primer
Primer Details for TRINITY_DN12775_c0_g3_i3 |
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| (CTCA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGTTGCGGCACTGTCATTT | ATATGGCATCGGGCTCATGG | Start: 2010 |
| Tm | 59.969 | 60.035 | End: 2196 |
| Size | 20 | 20 | 187 |
| Primer 2 details |
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| Sequence | TCTCTCCCTCTCTTTCGCGA | ACCACCGTGTACGAACTCAC | Start: 136 |
| Tm | 60.035 | 59.969 | End: 401 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
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| Sequence | TCTCTCCCTCTCTTTCGCGA | TCGTACAAGCGCACAGTCTT | Start: 136 |
| Tm | 60.035 | 59.968 | End: 358 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
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| Sequence | GTGAGTTCGTACACGGTGGT | TTGCCCCTCTAGGATCCCAA | Start: 382 |
| Tm | 59.969 | 59.954 | End: 615 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
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| Sequence | AAGACTGTGCGCTTGTACGA | TTGCCCCTCTAGGATCCCAA | Start: 339 |
| Tm | 59.968 | 59.954 | End: 615 |
| Size | 20 | 20 | 277 |
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