Primer Details for TRINITY_DN12773_c4_g1_i1 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCAAAAATCAAACAGATATGCGA | AGCGAGTTGTTTTGGTCCGA | Start: 6 |
| Tm | 58.047 | 60.179 | End: 133 |
| Size | 24 | 20 | 128 |
| Primer 2 details | |||
| Sequence | AGCAAAAATCAAACAGATATGCGA | GCGAGTTGTTTTGGTCCGAG | Start: 6 |
| Tm | 58.047 | 59.765 | End: 132 |
| Size | 24 | 20 | 127 |
| Primer 3 details | |||
| Sequence | AGCAAAAATCAAACAGATATGCGAA | AGCGAGTTGTTTTGGTCCGA | Start: 6 |
| Tm | 58.611 | 60.179 | End: 133 |
| Size | 25 | 20 | 128 |
| Primer 4 details | |||
| Sequence | AGCAAAAATCAAACAGATATGCGAA | GCGAGTTGTTTTGGTCCGAG | Start: 6 |
| Tm | 58.611 | 59.765 | End: 132 |
| Size | 25 | 20 | 127 |
| Primer 5 details | |||
| Sequence | GCAAAAATCAAACAGATATGCGAA | AGCGAGTTGTTTTGGTCCGA | Start: 7 |
| Tm | 57.369 | 60.179 | End: 133 |
| Size | 24 | 20 | 127 |
