Primer Details for TRINITY_DN12771_c0_g6_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCTGGTGCATGCTTTTGA | TCCTTTGCTGACAATAAAAGTTCT | Start: 55 |
| Tm | 60.203 | 57.04 | End: 277 |
| Size | 21 | 24 | 223 |
| Primer 2 details | |||
| Sequence | CCTCTGGTGCATGCTTTTGA | TCCTTTGCTGACAATAAAAGTTCT | Start: 56 |
| Tm | 58.75 | 57.04 | End: 277 |
| Size | 20 | 24 | 222 |
| Primer 3 details | |||
| Sequence | TCCTCTGGTGCATGCTTTTG | TCCTTTGCTGACAATAAAAGTTCT | Start: 55 |
| Tm | 58.75 | 57.04 | End: 277 |
| Size | 20 | 24 | 223 |
| Primer 4 details | |||
| Sequence | TCCTCTGGTGCATGCTTTTGA | TCCTTTGCTGACAATAAAAGTTCTTCA | Start: 55 |
| Tm | 60.203 | 59.932 | End: 277 |
| Size | 21 | 27 | 223 |
| Primer 5 details | |||
| Sequence | CCTCTGGTGCATGCTTTTGA | TCCTTTGCTGACAATAAAAGTTCTTCA | Start: 56 |
| Tm | 58.75 | 59.932 | End: 277 |
| Size | 20 | 27 | 222 |
