ssr_primer
Primer Details for TRINITY_DN12767_c4_g1_i2 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACAGCCGGACAAACAAGC | TGTGGAATCTGCCGCTGAAT | Start: 149 |
| Tm | 59.898 | 60.035 | End: 268 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | AAACAGCCGGACAAACAAGC | TAGTGTGGAATCTGCCGCTG | Start: 149 |
| Tm | 59.898 | 60.108 | End: 271 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | AGGCTTGGGGCGATACAAAT | ATCATTGTGGCTGCCTTCGA | Start: 887 |
| Tm | 59.741 | 60.035 | End: 1166 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | CAGCGGCAGATTCCACACTA | GCCCACATGATCTCTGGTGT | Start: 252 |
| Tm | 60.108 | 59.746 | End: 530 |
| Size | 20 | 20 | 279 |
| Primer 5 details |
|---|
| Sequence | ATTCAGCGGCAGATTCCACA | CAAGAAGATTTCGGCCGTCG | Start: 249 |
| Tm | 60.035 | 59.63 | End: 441 |
| Size | 20 | 20 | 193 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACAGCCGGACAAACAAGC | TGTGGAATCTGCCGCTGAAT | Start: 149 |
| Tm | 59.898 | 60.035 | End: 268 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | AAACAGCCGGACAAACAAGC | TAGTGTGGAATCTGCCGCTG | Start: 149 |
| Tm | 59.898 | 60.108 | End: 271 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | AGGCTTGGGGCGATACAAAT | ATCATTGTGGCTGCCTTCGA | Start: 887 |
| Tm | 59.741 | 60.035 | End: 1166 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | CAGCGGCAGATTCCACACTA | GCCCACATGATCTCTGGTGT | Start: 252 |
| Tm | 60.108 | 59.746 | End: 530 |
| Size | 20 | 20 | 279 |
| Primer 5 details |
|---|
| Sequence | ATTCAGCGGCAGATTCCACA | CAAGAAGATTTCGGCCGTCG | Start: 249 |
| Tm | 60.035 | 59.63 | End: 441 |
| Size | 20 | 20 | 193 |
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