ssr_primer
Primer Details for TRINITY_DN12761_c1_g1_i10 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTTCTTGTCTCGCTCGCA | GCACCCAGAAGAGGAGAACC | Start: 695 |
| Tm | 59.968 | 60.036 | End: 880 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | GGTTCTCCTCTTCTGGGTGC | GATGTGGCTGGGGGATTTCA | Start: 861 |
| Tm | 60.036 | 60.033 | End: 1137 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCTTCGTCTTCCTGGAACGC | Start: 4947 |
| Tm | 60.035 | 60.039 | End: 5063 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | TGAAATCCCCCAGCCACATC | TTTTGCCTCCATGGTGGGTT | Start: 1118 |
| Tm | 60.033 | 60.105 | End: 1264 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCGTCTTCCTGGAACGCAAA | Start: 4947 |
| Tm | 60.035 | 59.895 | End: 5060 |
| Size | 20 | 20 | 114 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTTCTTGTCTCGCTCGCA | GCACCCAGAAGAGGAGAACC | Start: 695 |
| Tm | 59.968 | 60.036 | End: 880 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | GGTTCTCCTCTTCTGGGTGC | GATGTGGCTGGGGGATTTCA | Start: 861 |
| Tm | 60.036 | 60.033 | End: 1137 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCTTCGTCTTCCTGGAACGC | Start: 4947 |
| Tm | 60.035 | 60.039 | End: 5063 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | TGAAATCCCCCAGCCACATC | TTTTGCCTCCATGGTGGGTT | Start: 1118 |
| Tm | 60.033 | 60.105 | End: 1264 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCGTCTTCCTGGAACGCAAA | Start: 4947 |
| Tm | 60.035 | 59.895 | End: 5060 |
| Size | 20 | 20 | 114 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTTCTTGTCTCGCTCGCA | GCACCCAGAAGAGGAGAACC | Start: 695 |
| Tm | 59.968 | 60.036 | End: 880 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | GGTTCTCCTCTTCTGGGTGC | GATGTGGCTGGGGGATTTCA | Start: 861 |
| Tm | 60.036 | 60.033 | End: 1137 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCTTCGTCTTCCTGGAACGC | Start: 4947 |
| Tm | 60.035 | 60.039 | End: 5063 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | TGAAATCCCCCAGCCACATC | TTTTGCCTCCATGGTGGGTT | Start: 1118 |
| Tm | 60.033 | 60.105 | End: 1264 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCGTCTTCCTGGAACGCAAA | Start: 4947 |
| Tm | 60.035 | 59.895 | End: 5060 |
| Size | 20 | 20 | 114 |
(ATG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTTCTTGTCTCGCTCGCA | GCACCCAGAAGAGGAGAACC | Start: 695 |
| Tm | 59.968 | 60.036 | End: 880 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | GGTTCTCCTCTTCTGGGTGC | GATGTGGCTGGGGGATTTCA | Start: 861 |
| Tm | 60.036 | 60.033 | End: 1137 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCTTCGTCTTCCTGGAACGC | Start: 4947 |
| Tm | 60.035 | 60.039 | End: 5063 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | TGAAATCCCCCAGCCACATC | TTTTGCCTCCATGGTGGGTT | Start: 1118 |
| Tm | 60.033 | 60.105 | End: 1264 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCGTCTTCCTGGAACGCAAA | Start: 4947 |
| Tm | 60.035 | 59.895 | End: 5060 |
| Size | 20 | 20 | 114 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTTCTTGTCTCGCTCGCA | GCACCCAGAAGAGGAGAACC | Start: 695 |
| Tm | 59.968 | 60.036 | End: 880 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | GGTTCTCCTCTTCTGGGTGC | GATGTGGCTGGGGGATTTCA | Start: 861 |
| Tm | 60.036 | 60.033 | End: 1137 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCTTCGTCTTCCTGGAACGC | Start: 4947 |
| Tm | 60.035 | 60.039 | End: 5063 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | TGAAATCCCCCAGCCACATC | TTTTGCCTCCATGGTGGGTT | Start: 1118 |
| Tm | 60.033 | 60.105 | End: 1264 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCGTCTTCCTGGAACGCAAA | Start: 4947 |
| Tm | 60.035 | 59.895 | End: 5060 |
| Size | 20 | 20 | 114 |
(AAG)6(AG)14*: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTTCTTGTCTCGCTCGCA | GCACCCAGAAGAGGAGAACC | Start: 695 |
| Tm | 59.968 | 60.036 | End: 880 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | GGTTCTCCTCTTCTGGGTGC | GATGTGGCTGGGGGATTTCA | Start: 861 |
| Tm | 60.036 | 60.033 | End: 1137 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCTTCGTCTTCCTGGAACGC | Start: 4947 |
| Tm | 60.035 | 60.039 | End: 5063 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | TGAAATCCCCCAGCCACATC | TTTTGCCTCCATGGTGGGTT | Start: 1118 |
| Tm | 60.033 | 60.105 | End: 1264 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | GCCTAGGGAAGCCGTTTTCT | TCGTCTTCCTGGAACGCAAA | Start: 4947 |
| Tm | 60.035 | 59.895 | End: 5060 |
| Size | 20 | 20 | 114 |
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