ssr_primer
Primer Details for TRINITY_DN12749_c1_g1_i2 |
|---|
| (AG)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGAAAAACGCCGAGACAGA | TCGCACAATCAGAGCACAGT | Start: 3862 |
| Tm | 59.969 | 59.966 | End: 4106 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | AGGACATGAATGGCAGGTGG | ACTGCATCTGGTCCATGTGG | Start: 2525 |
| Tm | 60.034 | 60.035 | End: 2732 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | GAGCCCCTCCACAGACTTTC | CATTTTTGTTCGCTGGCCCA | Start: 1789 |
| Tm | 60.036 | 59.967 | End: 1956 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | GGATGCACCTGAACACCAGA | ACTGCATCTGGTCCATGTGG | Start: 2500 |
| Tm | 59.963 | 60.035 | End: 2732 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | AGGACATGAATGGCAGGTGG | TGGCCACGAAGACGATATGG | Start: 2525 |
| Tm | 60.034 | 59.896 | End: 2664 |
| Size | 20 | 20 | 140 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGAAAAACGCCGAGACAGA | TCGCACAATCAGAGCACAGT | Start: 3862 |
| Tm | 59.969 | 59.966 | End: 4106 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | AGGACATGAATGGCAGGTGG | ACTGCATCTGGTCCATGTGG | Start: 2525 |
| Tm | 60.034 | 60.035 | End: 2732 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | GAGCCCCTCCACAGACTTTC | CATTTTTGTTCGCTGGCCCA | Start: 1789 |
| Tm | 60.036 | 59.967 | End: 1956 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | GGATGCACCTGAACACCAGA | ACTGCATCTGGTCCATGTGG | Start: 2500 |
| Tm | 59.963 | 60.035 | End: 2732 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | AGGACATGAATGGCAGGTGG | TGGCCACGAAGACGATATGG | Start: 2525 |
| Tm | 60.034 | 59.896 | End: 2664 |
| Size | 20 | 20 | 140 |
(CT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGAAAAACGCCGAGACAGA | TCGCACAATCAGAGCACAGT | Start: 3862 |
| Tm | 59.969 | 59.966 | End: 4106 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | AGGACATGAATGGCAGGTGG | ACTGCATCTGGTCCATGTGG | Start: 2525 |
| Tm | 60.034 | 60.035 | End: 2732 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | GAGCCCCTCCACAGACTTTC | CATTTTTGTTCGCTGGCCCA | Start: 1789 |
| Tm | 60.036 | 59.967 | End: 1956 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | GGATGCACCTGAACACCAGA | ACTGCATCTGGTCCATGTGG | Start: 2500 |
| Tm | 59.963 | 60.035 | End: 2732 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | AGGACATGAATGGCAGGTGG | TGGCCACGAAGACGATATGG | Start: 2525 |
| Tm | 60.034 | 59.896 | End: 2664 |
| Size | 20 | 20 | 140 |
(AG)6acgggggaagggagtaatcgattcttacaagaactccaacgaatttaagcggaagaattcctgtgatccaatt(GA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGAAAAACGCCGAGACAGA | TCGCACAATCAGAGCACAGT | Start: 3862 |
| Tm | 59.969 | 59.966 | End: 4106 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | AGGACATGAATGGCAGGTGG | ACTGCATCTGGTCCATGTGG | Start: 2525 |
| Tm | 60.034 | 60.035 | End: 2732 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | GAGCCCCTCCACAGACTTTC | CATTTTTGTTCGCTGGCCCA | Start: 1789 |
| Tm | 60.036 | 59.967 | End: 1956 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | GGATGCACCTGAACACCAGA | ACTGCATCTGGTCCATGTGG | Start: 2500 |
| Tm | 59.963 | 60.035 | End: 2732 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | AGGACATGAATGGCAGGTGG | TGGCCACGAAGACGATATGG | Start: 2525 |
| Tm | 60.034 | 59.896 | End: 2664 |
| Size | 20 | 20 | 140 |
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