Primer Details for TRINITY_DN12747_c2_g1_i1 | |||
|---|---|---|---|
| (A)17: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGATACTTCAGTTAAATGCACT | GCTGATGGGAAGGCTAACCA | Start: 76 |
| Tm | 57.933 | 59.744 | End: 286 |
| Size | 24 | 20 | 211 |
| Primer 2 details | |||
| Sequence | AGGGATACTTCAGTTAAATGCACT | AGCTGATGGGAAGGCTAACC | Start: 76 |
| Tm | 57.933 | 59.451 | End: 287 |
| Size | 24 | 20 | 212 |
| Primer 3 details | |||
| Sequence | AAGGGATACTTCAGTTAAATGCACT | GCTGATGGGAAGGCTAACCA | Start: 75 |
| Tm | 58.515 | 59.744 | End: 286 |
| Size | 25 | 20 | 212 |
| Primer 4 details | |||
| Sequence | TGGTAAAGAAAGGGATACTTCAGT | GCTGATGGGAAGGCTAACCA | Start: 66 |
| Tm | 57.302 | 59.744 | End: 286 |
| Size | 24 | 20 | 221 |
| Primer 5 details | |||
| Sequence | AAGGGATACTTCAGTTAAATGCAC | GCTGATGGGAAGGCTAACCA | Start: 75 |
| Tm | 57.241 | 59.744 | End: 286 |
| Size | 24 | 20 | 212 |
