ssr_primer
Primer Details for TRINITY_DN12729_c2_g3_i3 |
|---|
| (TA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCGGGACTCTTGAACTGC | TCTCGCAGTCCAAGCCAAAT | Start: 133 |
| Tm | 59.681 | 59.963 | End: 278 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
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| Sequence | AGTCGGGACTCTTGAACTGC | TCGCAGTCCAAGCCAAATCA | Start: 133 |
| Tm | 59.681 | 60.251 | End: 276 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
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| Sequence | GTCGGGACTCTTGAACTGCC | TCTCGCAGTCCAAGCCAAAT | Start: 134 |
| Tm | 60.671 | 59.963 | End: 278 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
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| Sequence | ATTTGGCTTGGACTGCGAGA | TGTGGCACGTCAGATTCAGA | Start: 259 |
| Tm | 59.963 | 59.321 | End: 368 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | AGTCGGGACTCTTGAACTGC | CTCGCAGTCCAAGCCAAATC | Start: 133 |
| Tm | 59.681 | 59.551 | End: 277 |
| Size | 20 | 20 | 145 |
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