ssr_primer
Primer Details for TRINITY_DN12725_c0_g1_i4 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGCCAATCACATCAGCA | GCACACGCACAACAAGATGT | Start: 2252 |
| Tm | 59.961 | 59.971 | End: 2497 |
| Size | 20 | 20 | 246 |
| Primer 2 details |
|---|
| Sequence | AAGAGTGGGGCACATGAAGG | CAAAACAGAGTCAGCGTGGC | Start: 344 |
| Tm | 59.961 | 60.041 | End: 507 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | ACATCTTGTTGTGCGTGTGC | ATGGTGCCTTGTTGGGTTCA | Start: 2478 |
| Tm | 59.971 | 60.106 | End: 2731 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | AAGAGTGGGGCACATGAAGG | CACTCCGAAGCTATGACGCT | Start: 344 |
| Tm | 59.961 | 59.899 | End: 559 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | ACACCATCGTCAAGCGTCAT | GCATAGCTCCACCTGAAGCA | Start: 528 |
| Tm | 60.037 | 60.108 | End: 801 |
| Size | 20 | 20 | 274 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGCCAATCACATCAGCA | GCACACGCACAACAAGATGT | Start: 2252 |
| Tm | 59.961 | 59.971 | End: 2497 |
| Size | 20 | 20 | 246 |
| Primer 2 details |
|---|
| Sequence | AAGAGTGGGGCACATGAAGG | CAAAACAGAGTCAGCGTGGC | Start: 344 |
| Tm | 59.961 | 60.041 | End: 507 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | ACATCTTGTTGTGCGTGTGC | ATGGTGCCTTGTTGGGTTCA | Start: 2478 |
| Tm | 59.971 | 60.106 | End: 2731 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | AAGAGTGGGGCACATGAAGG | CACTCCGAAGCTATGACGCT | Start: 344 |
| Tm | 59.961 | 59.899 | End: 559 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | ACACCATCGTCAAGCGTCAT | GCATAGCTCCACCTGAAGCA | Start: 528 |
| Tm | 60.037 | 60.108 | End: 801 |
| Size | 20 | 20 | 274 |
(A)10ccattgaaatggata(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGCCAATCACATCAGCA | GCACACGCACAACAAGATGT | Start: 2252 |
| Tm | 59.961 | 59.971 | End: 2497 |
| Size | 20 | 20 | 246 |
| Primer 2 details |
|---|
| Sequence | AAGAGTGGGGCACATGAAGG | CAAAACAGAGTCAGCGTGGC | Start: 344 |
| Tm | 59.961 | 60.041 | End: 507 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | ACATCTTGTTGTGCGTGTGC | ATGGTGCCTTGTTGGGTTCA | Start: 2478 |
| Tm | 59.971 | 60.106 | End: 2731 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | AAGAGTGGGGCACATGAAGG | CACTCCGAAGCTATGACGCT | Start: 344 |
| Tm | 59.961 | 59.899 | End: 559 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | ACACCATCGTCAAGCGTCAT | GCATAGCTCCACCTGAAGCA | Start: 528 |
| Tm | 60.037 | 60.108 | End: 801 |
| Size | 20 | 20 | 274 |
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