ssr_primer
Primer Details for TRINITY_DN12717_c1_g1_i1 |
|---|
| (G)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCACAAACGGAGAGGGTT | AGAGAGAGAGGGCATCCACC | Start: 95 |
| Tm | 60.107 | 60.398 | End: 290 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | TTTGCACAAACGGAGAGGGT | AGAGAGAGAGGGCATCCACC | Start: 94 |
| Tm | 60.107 | 60.398 | End: 290 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | GGGTCGCTGACTAGGGAAAG | AGAGAGAGAGGGCATCCACC | Start: 45 |
| Tm | 59.824 | 60.398 | End: 290 |
| Size | 20 | 20 | 246 |
| Primer 4 details |
|---|
| Sequence | GGGTCGCTGACTAGGGAAAG | ATGAGTAACCGGCCAAACCA | Start: 45 |
| Tm | 59.824 | 59.597 | End: 144 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | AAACGGAGAGGGTTCACGTG | AGAGAGAGAGGGCATCCACC | Start: 101 |
| Tm | 60.25 | 60.398 | End: 290 |
| Size | 20 | 20 | 190 |
(CT)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCACAAACGGAGAGGGTT | AGAGAGAGAGGGCATCCACC | Start: 95 |
| Tm | 60.107 | 60.398 | End: 290 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | TTTGCACAAACGGAGAGGGT | AGAGAGAGAGGGCATCCACC | Start: 94 |
| Tm | 60.107 | 60.398 | End: 290 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | GGGTCGCTGACTAGGGAAAG | AGAGAGAGAGGGCATCCACC | Start: 45 |
| Tm | 59.824 | 60.398 | End: 290 |
| Size | 20 | 20 | 246 |
| Primer 4 details |
|---|
| Sequence | GGGTCGCTGACTAGGGAAAG | ATGAGTAACCGGCCAAACCA | Start: 45 |
| Tm | 59.824 | 59.597 | End: 144 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | AAACGGAGAGGGTTCACGTG | AGAGAGAGAGGGCATCCACC | Start: 101 |
| Tm | 60.25 | 60.398 | End: 290 |
| Size | 20 | 20 | 190 |
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