ssr_primer
Primer Details for TRINITY_DN12703_c1_g2_i2 |
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| (T)11cttttgttcttttgcttcgccttgaatcgaaatccaaaatactcaaaatccttcgagaaaaaaagga(AAAC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGGTCAGTGGAAGCAGAG | GGGAAACTGGCGGAGAAGAA | Start: 648 |
| Tm | 59.966 | 59.964 | End: 800 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
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| Sequence | ATGCAGTGGAGGAACGGAAG | AACCGCCGTTCATCTTCCAT | Start: 1457 |
| Tm | 60.036 | 60.035 | End: 1586 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
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| Sequence | GAATGCAGTGGAGGAACGGA | AACCGCCGTTCATCTTCCAT | Start: 1455 |
| Tm | 60.036 | 60.035 | End: 1586 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
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| Sequence | TCACGTTATGCTCCACCACC | TGAACCGGCTTCATACCACC | Start: 1202 |
| Tm | 60.037 | 60.036 | End: 1435 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
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| Sequence | CCGGTCCAATTTCCTGTCGA | TGAACCGGCTTCATACCACC | Start: 1179 |
| Tm | 60.037 | 60.036 | End: 1435 |
| Size | 20 | 20 | 257 |
(T)29: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGGTCAGTGGAAGCAGAG | GGGAAACTGGCGGAGAAGAA | Start: 648 |
| Tm | 59.966 | 59.964 | End: 800 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | ATGCAGTGGAGGAACGGAAG | AACCGCCGTTCATCTTCCAT | Start: 1457 |
| Tm | 60.036 | 60.035 | End: 1586 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | GAATGCAGTGGAGGAACGGA | AACCGCCGTTCATCTTCCAT | Start: 1455 |
| Tm | 60.036 | 60.035 | End: 1586 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | TCACGTTATGCTCCACCACC | TGAACCGGCTTCATACCACC | Start: 1202 |
| Tm | 60.037 | 60.036 | End: 1435 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | CCGGTCCAATTTCCTGTCGA | TGAACCGGCTTCATACCACC | Start: 1179 |
| Tm | 60.037 | 60.036 | End: 1435 |
| Size | 20 | 20 | 257 |
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