ssr_primer
Primer Details for TRINITY_DN12681_c7_g6_i1 |
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| (TAAA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGACGTCACACTCTCCAAC | TCCTGCAAGAATCCGGCATT | Start: 1754 |
| Tm | 59.97 | 60.034 | End: 2025 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
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| Sequence | ACGACGTCACACTCTCCAAC | TTCCTGCAAGAATCCGGCAT | Start: 1754 |
| Tm | 59.97 | 60.034 | End: 2026 |
| Size | 20 | 20 | 273 |
| Primer 3 details |
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| Sequence | ACAGTTGAACCACGCATTGC | GGGCCACACTTCTTCTCGAA | Start: 803 |
| Tm | 59.969 | 59.965 | End: 944 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
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| Sequence | CAGACTGGCGGAGGTGATTT | GTTGGAGAGTGTGACGTCGT | Start: 1544 |
| Tm | 60.036 | 59.97 | End: 1773 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
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| Sequence | ACACCATTCTGCTCGTGAGG | GTTGGAGAGTGTGACGTCGT | Start: 1648 |
| Tm | 60.037 | 59.97 | End: 1773 |
| Size | 20 | 20 | 126 |
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