ssr_primer
Primer Details for TRINITY_DN12681_c4_g2_i1 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGACATAACAGAGCCCTA | TGGAACTGCATCTGCTTGCT | Start: 0 |
| Tm | 59.249 | 60.251 | End: 131 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
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| Sequence | GGCGACATAACAGAGCCCTA | ACGGTGGTTAAAAGCTCCCC | Start: 0 |
| Tm | 59.249 | 60.251 | End: 201 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
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| Sequence | GGCGACATAACAGAGCCCTA | GGTGGTTAAAAGCTCCCCCT | Start: 0 |
| Tm | 59.249 | 59.592 | End: 199 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
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| Sequence | GCGACATAACAGAGCCCTACA | TGGAACTGCATCTGCTTGCT | Start: 1 |
| Tm | 59.864 | 60.251 | End: 131 |
| Size | 21 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | GCGACATAACAGAGCCCTACA | ACGGTGGTTAAAAGCTCCCC | Start: 1 |
| Tm | 59.864 | 60.251 | End: 201 |
| Size | 21 | 20 | 201 |
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