ssr_primer
Primer Details for TRINITY_DN12680_c1_g2_i2 |
|---|
| (CT)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCAGCTGCTTATCTCGCT | ACACAGCCACATCACTACCG | Start: 904 |
| Tm | 59.896 | 60.037 | End: 1148 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CTGGATTTGCGGCTTTGCTT | AGCGAGATAAGCAGCTGACC | Start: 698 |
| Tm | 60.038 | 59.896 | End: 923 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | GGTCAGCTGCTTATCTCGCT | CCTTTGCACACAGCCACATC | Start: 904 |
| Tm | 59.896 | 60.039 | End: 1155 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | CTCCAACGCCTCGGAAAGAT | ACACAGCCACATCACTACCG | Start: 878 |
| Tm | 60.108 | 60.037 | End: 1148 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | CTGGATTTGCGGCTTTGCTT | ATCTTTCCGAGGCGTTGGAG | Start: 698 |
| Tm | 60.038 | 60.108 | End: 897 |
| Size | 20 | 20 | 200 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCAGCTGCTTATCTCGCT | ACACAGCCACATCACTACCG | Start: 904 |
| Tm | 59.896 | 60.037 | End: 1148 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CTGGATTTGCGGCTTTGCTT | AGCGAGATAAGCAGCTGACC | Start: 698 |
| Tm | 60.038 | 59.896 | End: 923 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | GGTCAGCTGCTTATCTCGCT | CCTTTGCACACAGCCACATC | Start: 904 |
| Tm | 59.896 | 60.039 | End: 1155 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | CTCCAACGCCTCGGAAAGAT | ACACAGCCACATCACTACCG | Start: 878 |
| Tm | 60.108 | 60.037 | End: 1148 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | CTGGATTTGCGGCTTTGCTT | ATCTTTCCGAGGCGTTGGAG | Start: 698 |
| Tm | 60.038 | 60.108 | End: 897 |
| Size | 20 | 20 | 200 |
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