ssr_primer
Primer Details for TRINITY_DN12675_c3_g2_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGATCCCCATGACCACAACA | TGGCCGGCTGAAATCATCTT | Start: 1768 |
| Tm | 60.036 | 60.034 | End: 1948 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
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| Sequence | GCTGCAAGAGTGGGAGGAAT | CTGCAACCCCTTCCTTCCAT | Start: 574 |
| Tm | 60.035 | 59.96 | End: 772 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
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| Sequence | CGATCCCCATGACCACAACA | ATGGCCCTCTTGGTTCCAAG | Start: 1768 |
| Tm | 60.036 | 59.96 | End: 1873 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
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| Sequence | CGATCCCCATGACCACAACA | TCTTGCATACTCGAGTGGCC | Start: 1768 |
| Tm | 60.036 | 59.824 | End: 1963 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
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| Sequence | TATGCTTGGGGTTGGCCAAT | TGTGGAGGGGCCTTCTAACT | Start: 1191 |
| Tm | 59.958 | 60.179 | End: 1416 |
| Size | 20 | 20 | 226 |
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