ssr_primer
Primer Details for TRINITY_DN12674_c2_g2_i2 |
|---|
| (T)37: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCTGGGAACTCTCCGGTA | GTCGCCAAACCAAAAGGACC | Start: 649 |
| Tm | 60.033 | 59.968 | End: 763 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | GAACCGGCCTTGAACCAAAC | TACCGGAGAGTTCCCAGGAG | Start: 471 |
| Tm | 59.968 | 60.033 | End: 668 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | ACCTGAGTGGCCCAAATAGC | GTTTGGTTCAAGGCCGGTTC | Start: 381 |
| Tm | 60.034 | 59.968 | End: 490 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | ACGGTTCCTTGCGAATGTCT | GTCGCCAAACCAAAAGGACC | Start: 590 |
| Tm | 59.965 | 59.968 | End: 763 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | GAACCGGCCTTGAACCAAAC | AGACATTCGCAAGGAACCGT | Start: 471 |
| Tm | 59.968 | 59.965 | End: 609 |
| Size | 20 | 20 | 139 |
(TC)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCTGGGAACTCTCCGGTA | GTCGCCAAACCAAAAGGACC | Start: 649 |
| Tm | 60.033 | 59.968 | End: 763 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | GAACCGGCCTTGAACCAAAC | TACCGGAGAGTTCCCAGGAG | Start: 471 |
| Tm | 59.968 | 60.033 | End: 668 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | ACCTGAGTGGCCCAAATAGC | GTTTGGTTCAAGGCCGGTTC | Start: 381 |
| Tm | 60.034 | 59.968 | End: 490 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | ACGGTTCCTTGCGAATGTCT | GTCGCCAAACCAAAAGGACC | Start: 590 |
| Tm | 59.965 | 59.968 | End: 763 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | GAACCGGCCTTGAACCAAAC | AGACATTCGCAAGGAACCGT | Start: 471 |
| Tm | 59.968 | 59.965 | End: 609 |
| Size | 20 | 20 | 139 |
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