ssr_primer
Primer Details for TRINITY_DN12670_c3_g9_i1 |
|---|
| (T)18gaaaaaccaaatgagactcattcaacttcacatcaaagtagagagaaaaaaaggaagaaattctaaaatctaatgaagagatgaaagtgggg(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAGGACGACGAATGAGTG | TGGCACCTTAAGGAACGTCC | Start: 954 |
| Tm | 60.179 | 59.964 | End: 1063 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | GTGGTGGTGGTCGAGGAAAT | GCCACTTTTGCAAGGCTTGT | Start: 977 |
| Tm | 59.964 | 60.179 | End: 1103 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | GGGAGGACGACGAATGAGTG | AAGGAACGTCCCTGTTGGTG | Start: 954 |
| Tm | 60.179 | 60.179 | End: 1054 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | GGGAGGACGACGAATGAGTG | GCCACTTTTGCAAGGCTTGT | Start: 954 |
| Tm | 60.179 | 60.179 | End: 1103 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | AGGGATCCGAGGTTCCTCTC | GTCAGCAGCAAGAAGACGGA | Start: 25 |
| Tm | 60.106 | 60.32 | End: 129 |
| Size | 20 | 20 | 105 |
(T)15ggctcagagaagtggaattttgttagggggaaaacaaagtgtaaacagacaaagagtaggtgatgaaacaagattatatttgagagta(CT)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAGGACGACGAATGAGTG | TGGCACCTTAAGGAACGTCC | Start: 954 |
| Tm | 60.179 | 59.964 | End: 1063 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | GTGGTGGTGGTCGAGGAAAT | GCCACTTTTGCAAGGCTTGT | Start: 977 |
| Tm | 59.964 | 60.179 | End: 1103 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | GGGAGGACGACGAATGAGTG | AAGGAACGTCCCTGTTGGTG | Start: 954 |
| Tm | 60.179 | 60.179 | End: 1054 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | GGGAGGACGACGAATGAGTG | GCCACTTTTGCAAGGCTTGT | Start: 954 |
| Tm | 60.179 | 60.179 | End: 1103 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | AGGGATCCGAGGTTCCTCTC | GTCAGCAGCAAGAAGACGGA | Start: 25 |
| Tm | 60.106 | 60.32 | End: 129 |
| Size | 20 | 20 | 105 |
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