ssr_primer
Primer Details for TRINITY_DN12665_c1_g1_i4 |
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| (A)10tcaattaatatttaatattgtaggtagtttaatggtaattatacttgtcaacgcccttctagagttctggtcatacttgtgta(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAATCCGCCATGACTGCTT | TGAAAGCAGCCTCCGGATTT | Start: 464 |
| Tm | 60.035 | 59.962 | End: 732 |
| Size | 20 | 20 | 269 |
| Primer 2 details |
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| Sequence | CGACACCGACTGGAAATGGA | AGGGCGCTCTACTATGGGAA | Start: 1983 |
| Tm | 60.038 | 60.106 | End: 2141 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
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| Sequence | TCGACACCGACTGGAAATGG | AGGGCGCTCTACTATGGGAA | Start: 1982 |
| Tm | 60.038 | 60.106 | End: 2141 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | TGAATCCGCCATGACTGCTT | TTGTTCCGCAACCTAGCCTT | Start: 464 |
| Tm | 60.035 | 59.89 | End: 666 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | TGAATCCGCCATGACTGCTT | GCCTCCGGATTTTGTCTTGC | Start: 464 |
| Tm | 60.035 | 59.829 | End: 724 |
| Size | 20 | 20 | 261 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAATCCGCCATGACTGCTT | TGAAAGCAGCCTCCGGATTT | Start: 464 |
| Tm | 60.035 | 59.962 | End: 732 |
| Size | 20 | 20 | 269 |
| Primer 2 details |
|---|
| Sequence | CGACACCGACTGGAAATGGA | AGGGCGCTCTACTATGGGAA | Start: 1983 |
| Tm | 60.038 | 60.106 | End: 2141 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | TCGACACCGACTGGAAATGG | AGGGCGCTCTACTATGGGAA | Start: 1982 |
| Tm | 60.038 | 60.106 | End: 2141 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | TGAATCCGCCATGACTGCTT | TTGTTCCGCAACCTAGCCTT | Start: 464 |
| Tm | 60.035 | 59.89 | End: 666 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | TGAATCCGCCATGACTGCTT | GCCTCCGGATTTTGTCTTGC | Start: 464 |
| Tm | 60.035 | 59.829 | End: 724 |
| Size | 20 | 20 | 261 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAATCCGCCATGACTGCTT | TGAAAGCAGCCTCCGGATTT | Start: 464 |
| Tm | 60.035 | 59.962 | End: 732 |
| Size | 20 | 20 | 269 |
| Primer 2 details |
|---|
| Sequence | CGACACCGACTGGAAATGGA | AGGGCGCTCTACTATGGGAA | Start: 1983 |
| Tm | 60.038 | 60.106 | End: 2141 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | TCGACACCGACTGGAAATGG | AGGGCGCTCTACTATGGGAA | Start: 1982 |
| Tm | 60.038 | 60.106 | End: 2141 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | TGAATCCGCCATGACTGCTT | TTGTTCCGCAACCTAGCCTT | Start: 464 |
| Tm | 60.035 | 59.89 | End: 666 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | TGAATCCGCCATGACTGCTT | GCCTCCGGATTTTGTCTTGC | Start: 464 |
| Tm | 60.035 | 59.829 | End: 724 |
| Size | 20 | 20 | 261 |
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