ssr_primer
Primer Details for TRINITY_DN12658_c1_g1_i5 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTACCCTGCTCAAACGGACA | ATTGCTGAGACAGAGGTGGC | Start: 1747 |
| Tm | 59.966 | 60.036 | End: 1958 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
|---|
| Sequence | GAGCCGGATCCTCACCAAAA | ATTGCTGAGACAGAGGTGGC | Start: 1769 |
| Tm | 60.035 | 60.036 | End: 1958 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | TCCAACAGACTTCCTTCGGC | GGAGCAAAGTAACGAGGGCT | Start: 5968 |
| Tm | 59.965 | 60.036 | End: 6134 |
| Size | 20 | 20 | 167 |
| Primer 4 details |
|---|
| Sequence | CAACAGACTTCCTTCGGCCT | GGAGCAAAGTAACGAGGGCT | Start: 5970 |
| Tm | 59.965 | 60.036 | End: 6134 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | AAGGAGGAAGATTGCAGGCC | CTTCGAAGACACCGGCTCTT | Start: 3063 |
| Tm | 60.034 | 60.038 | End: 3335 |
| Size | 20 | 20 | 273 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTACCCTGCTCAAACGGACA | ATTGCTGAGACAGAGGTGGC | Start: 1747 |
| Tm | 59.966 | 60.036 | End: 1958 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
|---|
| Sequence | GAGCCGGATCCTCACCAAAA | ATTGCTGAGACAGAGGTGGC | Start: 1769 |
| Tm | 60.035 | 60.036 | End: 1958 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | TCCAACAGACTTCCTTCGGC | GGAGCAAAGTAACGAGGGCT | Start: 5968 |
| Tm | 59.965 | 60.036 | End: 6134 |
| Size | 20 | 20 | 167 |
| Primer 4 details |
|---|
| Sequence | CAACAGACTTCCTTCGGCCT | GGAGCAAAGTAACGAGGGCT | Start: 5970 |
| Tm | 59.965 | 60.036 | End: 6134 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | AAGGAGGAAGATTGCAGGCC | CTTCGAAGACACCGGCTCTT | Start: 3063 |
| Tm | 60.034 | 60.038 | End: 3335 |
| Size | 20 | 20 | 273 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTACCCTGCTCAAACGGACA | ATTGCTGAGACAGAGGTGGC | Start: 1747 |
| Tm | 59.966 | 60.036 | End: 1958 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
|---|
| Sequence | GAGCCGGATCCTCACCAAAA | ATTGCTGAGACAGAGGTGGC | Start: 1769 |
| Tm | 60.035 | 60.036 | End: 1958 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | TCCAACAGACTTCCTTCGGC | GGAGCAAAGTAACGAGGGCT | Start: 5968 |
| Tm | 59.965 | 60.036 | End: 6134 |
| Size | 20 | 20 | 167 |
| Primer 4 details |
|---|
| Sequence | CAACAGACTTCCTTCGGCCT | GGAGCAAAGTAACGAGGGCT | Start: 5970 |
| Tm | 59.965 | 60.036 | End: 6134 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | AAGGAGGAAGATTGCAGGCC | CTTCGAAGACACCGGCTCTT | Start: 3063 |
| Tm | 60.034 | 60.038 | End: 3335 |
| Size | 20 | 20 | 273 |
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