ssr_primer
Primer Details for TRINITY_DN12656_c0_g1_i1 |
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| (AAAT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCTTGTCCGTTGCCCTTA | ACATTCACCGGGTCATTGGC | Start: 39 |
| Tm | 59.676 | 60.965 | End: 318 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
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| Sequence | CCTCTTGTCCGTTGCCCTTA | GGGTCATTGGCGGCATTAAT | Start: 39 |
| Tm | 59.676 | 58.96 | End: 309 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
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| Sequence | TGAACCAACGGCTTCATCCA | ACGTAAGACTGTCAGCATGCT | Start: 876 |
| Tm | 59.89 | 59.726 | End: 1012 |
| Size | 20 | 21 | 137 |
| Primer 4 details |
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| Sequence | GAGAGCCCTCTTGTCCGTTG | GGGTCATTGGCGGCATTAAT | Start: 33 |
| Tm | 60.39 | 58.96 | End: 309 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
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| Sequence | GCCAATGACCCGGTGAATGT | TGTGTGGTTTACTGCCAGGT | Start: 299 |
| Tm | 60.965 | 59.451 | End: 499 |
| Size | 20 | 20 | 201 |
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