ssr_primer
Primer Details for TRINITY_DN12653_c4_g7_i1 |
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| (ATT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCGTCCTCTCCCAATTTG | AGTTTGGGAAGGGCCAACAT | Start: 243 |
| Tm | 60.322 | 59.809 | End: 516 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
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| Sequence | TTGCCGTCCTCTCCCAATTT | AGTTTGGGAAGGGCCAACAT | Start: 242 |
| Tm | 59.596 | 59.809 | End: 516 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
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| Sequence | TTGCTATTGCCGTCCTCTCC | TTTGGGAAGGGCCAACATCT | Start: 236 |
| Tm | 59.822 | 59.513 | End: 514 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
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| Sequence | TTTTGGCACACTTGGAACGG | AGCAATCCAAGAGGCTGCAA | Start: 2373 |
| Tm | 59.542 | 60.251 | End: 2610 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
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| Sequence | TGGTAGCAGAGCTCTAGGGT | AGACGAGCAAAGTGGCAGAA | Start: 1796 |
| Tm | 59.368 | 59.893 | End: 1976 |
| Size | 20 | 20 | 181 |
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