ssr_primer
Primer Details for TRINITY_DN12651_c1_g1_i1 |
|---|
| (T)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGACTTGCACCCAACAGAG | AGCACGAAACTCATGTACCCA | Start: 38 |
| Tm | 60.179 | 59.654 | End: 266 |
| Size | 20 | 21 | 229 |
| Primer 2 details |
|---|
| Sequence | TGGACTTGCACCCAACAGAG | AGCAGTTTCCTCTCCACAACA | Start: 38 |
| Tm | 60.179 | 59.51 | End: 163 |
| Size | 20 | 21 | 126 |
| Primer 3 details |
|---|
| Sequence | CACCCAACAGAGCCATGGTA | AGCACGAAACTCATGTACCCA | Start: 46 |
| Tm | 59.672 | 59.654 | End: 266 |
| Size | 20 | 21 | 221 |
| Primer 4 details |
|---|
| Sequence | GTGGACTTGCACCCAACAGA | AGCACGAAACTCATGTACCCA | Start: 37 |
| Tm | 60.465 | 59.654 | End: 266 |
| Size | 20 | 21 | 230 |
| Primer 5 details |
|---|
| Sequence | TCTGTGGACTTGCACCCAAC | AGCACGAAACTCATGTACCCA | Start: 34 |
| Tm | 60.465 | 59.654 | End: 266 |
| Size | 20 | 21 | 233 |
(T)10aaagttatcaaaaggtaccaaacttacaatgtag(T)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGACTTGCACCCAACAGAG | AGCACGAAACTCATGTACCCA | Start: 38 |
| Tm | 60.179 | 59.654 | End: 266 |
| Size | 20 | 21 | 229 |
| Primer 2 details |
|---|
| Sequence | TGGACTTGCACCCAACAGAG | AGCAGTTTCCTCTCCACAACA | Start: 38 |
| Tm | 60.179 | 59.51 | End: 163 |
| Size | 20 | 21 | 126 |
| Primer 3 details |
|---|
| Sequence | CACCCAACAGAGCCATGGTA | AGCACGAAACTCATGTACCCA | Start: 46 |
| Tm | 59.672 | 59.654 | End: 266 |
| Size | 20 | 21 | 221 |
| Primer 4 details |
|---|
| Sequence | GTGGACTTGCACCCAACAGA | AGCACGAAACTCATGTACCCA | Start: 37 |
| Tm | 60.465 | 59.654 | End: 266 |
| Size | 20 | 21 | 230 |
| Primer 5 details |
|---|
| Sequence | TCTGTGGACTTGCACCCAAC | AGCACGAAACTCATGTACCCA | Start: 34 |
| Tm | 60.465 | 59.654 | End: 266 |
| Size | 20 | 21 | 233 |
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