ssr_primer
Primer Details for TRINITY_DN12649_c3_g4_i1 |
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| (T)10gtgtgattatattgttttttaaccctcca(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCCCCATAGCACCCTTA | TCCCCATCCAATGAGTGAAGC | Start: 30 |
| Tm | 60.253 | 60.064 | End: 225 |
| Size | 20 | 21 | 196 |
| Primer 2 details |
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| Sequence | ACACCCCCATAGCACCCTTA | CCCCATCCAATGAGTGAAGCA | Start: 30 |
| Tm | 60.253 | 60.341 | End: 224 |
| Size | 20 | 21 | 195 |
| Primer 3 details |
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| Sequence | GCACCCGGACATCTCAACTA | TCCCCATCCAATGAGTGAAGC | Start: 5 |
| Tm | 59.465 | 60.064 | End: 225 |
| Size | 20 | 21 | 221 |
| Primer 4 details |
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| Sequence | ACACCCCCATAGCACCCTTA | CCCCATCCAATGAGTGAAGC | Start: 30 |
| Tm | 60.253 | 58.603 | End: 224 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
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| Sequence | GTTGACACCCCCATAGCACC | TCCCCATCCAATGAGTGAAGC | Start: 26 |
| Tm | 60.68 | 60.064 | End: 225 |
| Size | 20 | 21 | 200 |
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