ssr_primer
Primer Details for TRINITY_DN12642_c1_g2_i4 |
|---|
| (AGAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACATCCCTCTTCGCACGT | TATCTGCGTGTGAGGGGAGA | Start: 64 |
| Tm | 59.965 | 60.034 | End: 209 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | AAACATCCCTCTTCGCACGT | TCTATCTGCGTGTGAGGGGA | Start: 64 |
| Tm | 59.965 | 60.034 | End: 211 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | GCCCCTGGTAAGCAGTCTTT | AATTCATCCCAGGGGCAAGG | Start: 3629 |
| Tm | 59.962 | 60.032 | End: 3844 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | TCCCCTCACACGCAGATAGA | TCCTTGGACATGTGGCACTC | Start: 192 |
| Tm | 60.034 | 59.963 | End: 357 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | TCTCCCCTCACACGCAGATA | TCCTTGGACATGTGGCACTC | Start: 190 |
| Tm | 60.034 | 59.963 | End: 357 |
| Size | 20 | 20 | 168 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACATCCCTCTTCGCACGT | TATCTGCGTGTGAGGGGAGA | Start: 64 |
| Tm | 59.965 | 60.034 | End: 209 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | AAACATCCCTCTTCGCACGT | TCTATCTGCGTGTGAGGGGA | Start: 64 |
| Tm | 59.965 | 60.034 | End: 211 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | GCCCCTGGTAAGCAGTCTTT | AATTCATCCCAGGGGCAAGG | Start: 3629 |
| Tm | 59.962 | 60.032 | End: 3844 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | TCCCCTCACACGCAGATAGA | TCCTTGGACATGTGGCACTC | Start: 192 |
| Tm | 60.034 | 59.963 | End: 357 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | TCTCCCCTCACACGCAGATA | TCCTTGGACATGTGGCACTC | Start: 190 |
| Tm | 60.034 | 59.963 | End: 357 |
| Size | 20 | 20 | 168 |
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