ssr_primer
Primer Details for TRINITY_DN12609_c1_g3_i5 |
|---|
| (GCA)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGAACCCCACCTTTTGCT | GCTGCCACCAATAAGGGACT | Start: 2780 |
| Tm | 60.032 | 60.034 | End: 3018 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
|---|
| Sequence | TTGGAACCCCACCTTTTGCT | AGCTGCCACCAATAAGGGAC | Start: 2780 |
| Tm | 60.032 | 60.034 | End: 3019 |
| Size | 20 | 20 | 240 |
| Primer 3 details |
|---|
| Sequence | AGCGGGGACATTAACACCAG | TTGCATTGGGGTCAGGCTAG | Start: 4065 |
| Tm | 60.035 | 60.034 | End: 4254 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | GAGCGGGGACATTAACACCA | TTGCATTGGGGTCAGGCTAG | Start: 4064 |
| Tm | 60.036 | 60.034 | End: 4254 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | GTCCATGGAAAGTCCCCAGG | AACTGCTCTTCCTTGGCTCC | Start: 2001 |
| Tm | 60.034 | 59.963 | End: 2207 |
| Size | 20 | 20 | 207 |
(CAG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGAACCCCACCTTTTGCT | GCTGCCACCAATAAGGGACT | Start: 2780 |
| Tm | 60.032 | 60.034 | End: 3018 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
|---|
| Sequence | TTGGAACCCCACCTTTTGCT | AGCTGCCACCAATAAGGGAC | Start: 2780 |
| Tm | 60.032 | 60.034 | End: 3019 |
| Size | 20 | 20 | 240 |
| Primer 3 details |
|---|
| Sequence | AGCGGGGACATTAACACCAG | TTGCATTGGGGTCAGGCTAG | Start: 4065 |
| Tm | 60.035 | 60.034 | End: 4254 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | GAGCGGGGACATTAACACCA | TTGCATTGGGGTCAGGCTAG | Start: 4064 |
| Tm | 60.036 | 60.034 | End: 4254 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | GTCCATGGAAAGTCCCCAGG | AACTGCTCTTCCTTGGCTCC | Start: 2001 |
| Tm | 60.034 | 59.963 | End: 2207 |
| Size | 20 | 20 | 207 |
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