ssr_primer
Primer Details for TRINITY_DN12609_c1_g1_i20 |
|---|
| (GCA)9: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGGACTCGCTGATTCTAC | CGGCTCCATATGCACTCCAT | Start: 242 |
| Tm | 59.969 | 59.964 | End: 428 |
| Size | 20 | 20 | 187 |
| Primer 2 details |
|---|
| Sequence | TGTCGTTCTTGGTCCACTCG | CGGCTCCATATGCACTCCAT | Start: 200 |
| Tm | 59.968 | 59.964 | End: 428 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GTGGCTTGTGTTTGGAGCAG | ACATTCCTCTGCACCAGTCG | Start: 1567 |
| Tm | 59.969 | 60.037 | End: 1738 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | ATGGAGTGCATATGGAGCCG | AAGAGCTCTTTGTGACCCCG | Start: 409 |
| Tm | 59.964 | 59.965 | End: 685 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | ACACTGCAGATTGCCCTCTC | ACATTCCTCTGCACCAGTCG | Start: 1638 |
| Tm | 60.036 | 60.037 | End: 1738 |
| Size | 20 | 20 | 101 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGGACTCGCTGATTCTAC | CGGCTCCATATGCACTCCAT | Start: 242 |
| Tm | 59.969 | 59.964 | End: 428 |
| Size | 20 | 20 | 187 |
| Primer 2 details |
|---|
| Sequence | TGTCGTTCTTGGTCCACTCG | CGGCTCCATATGCACTCCAT | Start: 200 |
| Tm | 59.968 | 59.964 | End: 428 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GTGGCTTGTGTTTGGAGCAG | ACATTCCTCTGCACCAGTCG | Start: 1567 |
| Tm | 59.969 | 60.037 | End: 1738 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | ATGGAGTGCATATGGAGCCG | AAGAGCTCTTTGTGACCCCG | Start: 409 |
| Tm | 59.964 | 59.965 | End: 685 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | ACACTGCAGATTGCCCTCTC | ACATTCCTCTGCACCAGTCG | Start: 1638 |
| Tm | 60.036 | 60.037 | End: 1738 |
| Size | 20 | 20 | 101 |
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