ssr_primer
Primer Details for TRINITY_DN12606_c0_g3_i5 |
|---|
| (A)10caaatgcttagagaattcttaaatctaaagaaaaacaacttttctt(A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGTCGCCAAAGCTGCAAA | CAGCTTGCCCTGGATAACCA | Start: 1809 |
| Tm | 59.969 | 60.034 | End: 1938 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
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| Sequence | GTGTTGGTTATGGGGCTGGA | TTTGCAGCTTTGGCGACTTC | Start: 1716 |
| Tm | 59.961 | 59.969 | End: 1828 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | TGGTACCAAATCGGACAGGC | AAGCTTTCCCCCTTCGATGG | Start: 633 |
| Tm | 60.036 | 60.034 | End: 854 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | TGGTACCAAATCGGACAGGC | CCAAGCTTTCCCCCTTCGAT | Start: 633 |
| Tm | 60.036 | 60.034 | End: 856 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | GTGAATGTGACCGTCGGAGT | CTCTCCCCCGCGTTTATCTC | Start: 2440 |
| Tm | 60.039 | 59.968 | End: 2588 |
| Size | 20 | 20 | 149 |
(CTT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGTCGCCAAAGCTGCAAA | CAGCTTGCCCTGGATAACCA | Start: 1809 |
| Tm | 59.969 | 60.034 | End: 1938 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
|---|
| Sequence | GTGTTGGTTATGGGGCTGGA | TTTGCAGCTTTGGCGACTTC | Start: 1716 |
| Tm | 59.961 | 59.969 | End: 1828 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | TGGTACCAAATCGGACAGGC | AAGCTTTCCCCCTTCGATGG | Start: 633 |
| Tm | 60.036 | 60.034 | End: 854 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | TGGTACCAAATCGGACAGGC | CCAAGCTTTCCCCCTTCGAT | Start: 633 |
| Tm | 60.036 | 60.034 | End: 856 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | GTGAATGTGACCGTCGGAGT | CTCTCCCCCGCGTTTATCTC | Start: 2440 |
| Tm | 60.039 | 59.968 | End: 2588 |
| Size | 20 | 20 | 149 |
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