ssr_primer
Primer Details for TRINITY_DN12604_c1_g4_i8 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACGACGGGAGACTTTTGG | TACGCTGTCCATTCCACACC | Start: 43 |
| Tm | 59.967 | 60.037 | End: 157 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | CCTGCTAAGAGCTGCCAGTT | GAGAGGGATGCCAATCTCCG | Start: 502 |
| Tm | 60.036 | 59.965 | End: 628 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TCGCAGAGACCAAGGAACAC | GGCCTGCTTTGTACTCACCT | Start: 2228 |
| Tm | 59.966 | 59.963 | End: 2328 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | CTCCACTCCATAGTCCACGC | GGCCTGCTTTGTACTCACCT | Start: 2177 |
| Tm | 59.897 | 59.963 | End: 2328 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | AGATGCTGCTGGCCATGAAT | GAGAGGGATGCCAATCTCCG | Start: 460 |
| Tm | 60.106 | 59.965 | End: 628 |
| Size | 20 | 20 | 169 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACGACGGGAGACTTTTGG | TACGCTGTCCATTCCACACC | Start: 43 |
| Tm | 59.967 | 60.037 | End: 157 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | CCTGCTAAGAGCTGCCAGTT | GAGAGGGATGCCAATCTCCG | Start: 502 |
| Tm | 60.036 | 59.965 | End: 628 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TCGCAGAGACCAAGGAACAC | GGCCTGCTTTGTACTCACCT | Start: 2228 |
| Tm | 59.966 | 59.963 | End: 2328 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | CTCCACTCCATAGTCCACGC | GGCCTGCTTTGTACTCACCT | Start: 2177 |
| Tm | 59.897 | 59.963 | End: 2328 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | AGATGCTGCTGGCCATGAAT | GAGAGGGATGCCAATCTCCG | Start: 460 |
| Tm | 60.106 | 59.965 | End: 628 |
| Size | 20 | 20 | 169 |
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