ssr_primer
Primer Details for TRINITY_DN12601_c3_g4_i5 |
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| (GAG)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCGACGAAGCATAGCTAT | CTCTGACGGACGAAGAGACG | Start: 656 |
| Tm | 60.038 | 59.904 | End: 807 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
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| Sequence | ACCCAGGGGATAAGAGGCAT | ACGCTAGTGGTTGAAGGCAA | Start: 1363 |
| Tm | 60.029 | 59.892 | End: 1625 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
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| Sequence | AGGCATCCCCATTCCCAAAG | ACGCTAGTGGTTGAAGGCAA | Start: 1377 |
| Tm | 60.032 | 59.892 | End: 1625 |
| Size | 20 | 20 | 249 |
| Primer 4 details |
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| Sequence | GAGGCATCCCCATTCCCAAA | ACGCTAGTGGTTGAAGGCAA | Start: 1376 |
| Tm | 60.032 | 59.892 | End: 1625 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
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| Sequence | TCTCCAACCTCCTCCACACA | GCCGGGAAGATCAAGACCAA | Start: 909 |
| Tm | 60.105 | 60.035 | End: 1075 |
| Size | 20 | 20 | 167 |
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