Primer Details for TRINITY_DN12591_c1_g1_i1 |
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| (AAAT)5(AT)9*: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTGCGGCTAAGAGGAATT | ACCTTACTGTTGTTTGTCATTTCCT | Start: 29 |
| Tm | 60.462 | 59.108 | End: 158 |
| Size | 20 | 25 | 130 |
| Primer 2 details |
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| Sequence | ATGCCTATATCTCCGTGCGG | ACCTTACTGTTGTTTGTCATTTCCT | Start: 17 |
| Tm | 59.467 | 59.108 | End: 158 |
| Size | 20 | 25 | 142 |
| Primer 3 details |
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| Sequence | CCGTGCGGCTAAGAGGAATT | ACCTTACTGTTGTTTGTCATTTCC | Start: 29 |
| Tm | 60.462 | 57.856 | End: 158 |
| Size | 20 | 24 | 130 |
| Primer 4 details |
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| Sequence | ATGCCTATATCTCCGTGCGG | ACCTTACTGTTGTTTGTCATTTCC | Start: 17 |
| Tm | 59.467 | 57.856 | End: 158 |
| Size | 20 | 24 | 142 |
| Primer 5 details |
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| Sequence | TATCTCCGTGCGGCTAAGAG | ACCTTACTGTTGTTTGTCATTTCCT | Start: 24 |
| Tm | 59.048 | 59.108 | End: 158 |
| Size | 20 | 25 | 135 |
