Primer Details for TRINITY_DN12589_c0_g2_i1 |
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| (TC)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATAGACTTGCATCGATCACCCC | TGGAGACAACGAAGAACTTCA | Start: 181 |
| Tm | 59.962 | 57.188 | End: 280 |
| Size | 22 | 21 | 100 |
| Primer 2 details |
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| Sequence | TGCAAGTTCATGCTTAGATAGAGAC | GGGGTGATCGATGCAAGTCT | Start: 77 |
| Tm | 58.955 | 59.821 | End: 202 |
| Size | 25 | 20 | 126 |
| Primer 3 details |
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| Sequence | TGCAAGTTCATGCTTAGATAGAGAC | AGGGGTGATCGATGCAAGTC | Start: 77 |
| Tm | 58.955 | 59.821 | End: 203 |
| Size | 25 | 20 | 127 |
| Primer 4 details |
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| Sequence | TGCAAGTTCATGCTTAGATAGAGAC | GAGGGGTGATCGATGCAAGT | Start: 77 |
| Tm | 58.955 | 59.821 | End: 204 |
| Size | 25 | 20 | 128 |
| Primer 5 details |
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| Sequence | TGCAAGTTCATGCTTAGATAGAGACT | GGGGTGATCGATGCAAGTCT | Start: 77 |
| Tm | 60.127 | 59.821 | End: 202 |
| Size | 26 | 20 | 126 |
